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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in many tumors, which leads to chromosome instability, and thus might add to tumorigenesis. This protein was uncovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important position in selling cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are already described. Two added splice variants have been noted but comprehensive nucleotide sequence details is just not nonetheless readily available.
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Blocking peptides are peptides that bind specially for the focus on antibody and block antibody binding. These peptide usually is made up of the epitope recognized with the antibody.
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This guide outlines further probable optimization of mobile and immunoassay parameters to be certain the best possible results are...
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This guidebook outlines more attainable optimization of mobile and immunoassay parameters to be sure the absolute best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By evaluating the staining from your blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs into the really conserved cyclin spouse and children, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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